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Learning aim

We will give you details of all the different steps of ultramicrotomy:

Abstract

France BioImaging Training - MOOC Series - ULTRAMICROTOMY AT ROOM TEMPERATURE - TEXTE TEASER L’ultramicrotomie est une étape essentielle pour tout microscopiste électronique ! En effet, la plupart des échantillons biologiques sont bien trop épais pour être observés tels quels au microscope électronique en transmission. Il est donc indispensable de réaliser des coupes de ces échantillons…

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France BioImaging Training - MOOC Series - ULTRAMICROTOMY AT ROOM TEMPERATURE - TEXTE TEASER L’ultramicrotomie est une étape essentielle pour tout microscopiste électronique ! En effet, la plupart des échantillons biologiques sont bien trop épais pour être observés tels quels au microscope électronique en transmission. Il est donc indispensable de réaliser des coupes de ces échantillons pour pouvoir les observer au MET. La formation à l’ultramicrotomie nécessite beaucoup de pratique, ce MOOC vous donnera les bases théoriques pour aborder cette technique et sera un précieux support lors de la mise en pratique sur l’ultramicrotome. Nous allons donc vous présenter dans ce MOOC toutes les étapes nécessaires à la réalisation de coupes. Nous allons parler des coupes d’échantillons une fois qu’ils…

Ontology context

Keywords

Learning aim

The sample block must be prepared in a way that the area of interest is on the surface of the block in a small area of about 1 X 1 mm or even smaller.…

Abstract

3 RAW Trimming The sample block must be prepared in a way that the area of interest is on the surface of the block in a small area of about 1 X 1 mm or even smaller. The block containing the sample will be ready to be finely trimmed and polished for ultra-thin sectioning.

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RAW Trimming Objective The sample block must be prepared in a way that the area of interest is on the surface of the block in a small area of about 1 X 1 mm or even smaller. The block containing the sample will be ready to be finely trimmed and polished for ultra-thin sectioning. General notions Samples for ultramicrotomy are generally included in the Epon resin which cures the sample and sample block. A saw (cf. Figure 3.2.1) can be used in order to separate the sample block in different parts, to remove big portions of empty resin or to reach the core part of a tissue of interest. Sample block can be sawn with a jewelers saw Afterwards the…

Ontology context

Keywords

Learning aim

The goal of fine trimming is to polish the sample surface and to trim the four sides of the pyramid to obtain a very small cutting area that allows the cutting of ultrathin sections.

Abstract

4 Fine trimming The goal of fine trimming is to polish the sample surface and to trim the four sides of the pyramid to obtain a very small cutting area that allows the cutting of ultrathin sections. In the first step of fine trimming, the sample surface should be polished and reflect light like a mirror.…

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Fine trimming Objective The goal of fine trimming is to polish the sample surface and to trim the four sides of the pyramid to obtain a very small cutting area that allows the cutting of ultrathin sections. General notions In the first step of fine trimming, the sample surface should be polished and reflect light like a mirror. This will help later with alignment of the blockface to the knife edge. In the second step of fine trimming, the four sides are shaped to form a pyramid with slopes of 45° to 60°. Top and bottom edges of the sample surface must be parallel. The top of the pyramid (or the blockface) has the shape of a trapezoid, a rectangle…

Ontology context

Keywords